Evaluation of chicken kidney and chicken embryo kidney cultures for the large-scale growth of attenuated influenza virus master strain A/Ann/Arbor/6/60- ca
Identifieur interne : 002467 ( Main/Exploration ); précédent : 002466; suivant : 002468Evaluation of chicken kidney and chicken embryo kidney cultures for the large-scale growth of attenuated influenza virus master strain A/Ann/Arbor/6/60- ca
Auteurs : G. A. Tannock [Australie] ; Deborah A. Bryce [Australie] ; Judith A. Paul [Australie]Source :
- Vaccine [ 0264-410X ] ; 1985.
English descriptors
- KwdEn :
- Teeft :
- Academic press, Allantoic, Allantoic cavity, Allantoicgrown preparations, Assay, Attenuated, Attenuated recombinants, Bead suspension, Bench centrifuge, Bovine kidney epithelial cells, Cell concentration, Cell culture, Cell growth, Cell suspensions, Cell volume, Cell volumes, Chick, Chick embryo, Chick embryos, Chicken embryo kidney, Chicken kidney, Chicken kidneys, Comparable yields, Culture fluid, Culture medium, Diploid cell line, Efficient growth, Embryo, Embryonated eggs, Growth curves, Higher yields, Identical doses, Immunogenicity, Infectious unit, Infectivity titres, Influenza, Influenza vaccines, Influenza virus, Influenza viruses, Initial suspension, Intranasal route, Kidney, Kidney pair, Large numbers, Less protein, Liquid nitrogen, Maintenance medium, Microcarrier, Microcarrier cultures, Microcarrier suspensions, Microcarriers, Monkey kidney cells, Monolayer, Monolayer cultures, Monolayers, Nuclei counts, Overnight trypsinization, Parental virus, Petri, Petri dish cultures, Plaque assays, Primary cells, Relative immunogenicity, Roller, Roller cultures, Same dilution, Separate experiments, Sodium bicarbonate, Standard error, Supernatant, Supernatant fluid, Support growth, Tannock, Total yields, Trypsin, Trypsin solution, Trypsinization, Trypsinized, Vaccine, Various intervals, Virus, Virus assay, Whole embryos.
Abstract
Primary chicken kidney (CK) and chicken embryo kidney (CEK) cells were evaluated as possible substrates for growth of the cold-adapted attenuated influenza vaccine master strain A/Ann Arbor/6/60 (A/AA/6/60-ca). Yields of 106–107 TCID50 per ml of culture fluid were obtained in either cell type. Yields from the human diploid strain MRC-5 were ∼100-fold less. More reproducible cultures were obtained from CEK cells, using an overnight trypsinization step at 4°C than from CK cells. Comparable yields per embryo were obtained from CEK cells grown in roller cultures to those grown on the surface of microcarriers. These yields were less than those obtained from the allantoic fluids of whole embryos. Frozen storage of CEK or CK cells, after primary trypsinization, dispersal from a cultured CK primary monolayer or culture on microcarriers, was unsuccessful The cold-adapted phenotype of A/AA/6/60-ca was retained after growth in CEK cultures and no differences in immunogenicity were detectable in mice between CEK- and allantoic-grown virus. Allantoic-grown preparations of A/AA/6/60-ca contained a lower protein concentration per infectious unit than those grown in CEK
Url:
DOI: 10.1016/S0264-410X(85)90245-2
Affiliations:
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Yields of 106–107 TCID50 per ml of culture fluid were obtained in either cell type. Yields from the human diploid strain MRC-5 were ∼100-fold less. More reproducible cultures were obtained from CEK cells, using an overnight trypsinization step at 4°C than from CK cells. Comparable yields per embryo were obtained from CEK cells grown in roller cultures to those grown on the surface of microcarriers. These yields were less than those obtained from the allantoic fluids of whole embryos. Frozen storage of CEK or CK cells, after primary trypsinization, dispersal from a cultured CK primary monolayer or culture on microcarriers, was unsuccessful The cold-adapted phenotype of A/AA/6/60-ca was retained after growth in CEK cultures and no differences in immunogenicity were detectable in mice between CEK- and allantoic-grown virus. Allantoic-grown preparations of A/AA/6/60-ca contained a lower protein concentration per infectious unit than those grown in CEK</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Tannock, G A" sort="Tannock, G A" uniqKey="Tannock G" first="G. A." last="Tannock">G. A. Tannock</name></noRegion><name sortKey="Bryce, Deborah A" sort="Bryce, Deborah A" uniqKey="Bryce D" first="Deborah A." last="Bryce">Deborah A. Bryce</name><name sortKey="Paul, Judith A" sort="Paul, Judith A" uniqKey="Paul J" first="Judith A." last="Paul">Judith A. Paul</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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